AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuale Utente Scaricare il pdf (Pagina 3)

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December 16, 2004 3:56 pm, GSG_Workflow.fm

Before You Begin

Chapter 1

Designing Primers

and Probes for

Quantification

Assays

Chapter 2

Designing Primers

and Probes for

Allelic Discrimination

Assays

Chapter 3

Ordering Primers

and Probes

Chapter 4

Introduction

Overview

Installing the

Software

Automatically Designing

Primers and Probes

Ordering the Selected

Primers and Probes

Basics

Manually Designing

Primers and Probes

Automatically Designing

Primers for

SYBR

Green Assays

Manually Designing

Primers for

SYBR

Green Assays

Overview

Automatically

Designing Primers

and Probes

Manually

Designing Primers

and Probes

Primer Express Software Version 3.0 Getting Started Guide iii

Primer Express

Software Version 3.0 Work-

flow

1 2 3 4 5 6 7 8 ... 51 52

Getting Started Guide 1
Chapter 1 3
Chapter 2 3
Chapter 3 3
Chapter 4 3
Contents 4
How to Use This Guide 6
How to Obtain Support 7
Before You Begin 8
Introduction 10
Installing the Software 11
Chapter 1 Before You Begin 12
Quantification Assays 14
Overview 15
To load a sequence file: 16
Finding Primers 17
Document 19
Ordering Primers 19
Saving the 19
If primers were not found: 22
To design the Reverse Primer: 24
Green Dye 26
Green Dye Assays 27
Allelic Discrimination Assays 34
Assigning a SNP 37
To design the Forward Primer: 44
Ordering Primers and Probes 46
Part Number 4362460 Rev. B 52

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuale Utente Pagina 3

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